NEW YORK (AP) ― Scientists have reached farther back than ever into the ancestry of humans to recover and analyze DNA, using a bone found in Spain that’s estimated to be 400,000 years old. So far, the achievement has provided more questions than answers about our ancient forerunners.

The feat surpasses the previous age record of about 100,000 years for genetic material recovered from members of the human evolutionary line. Older DNA has been mapped from animals.

Experts said the work shows that new techniques for working with ancient DNA may lead to more discoveries about human origins.

Results were presented online Wednesday in the journal Nature by Matthias Meyer and colleagues at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, with co-authors in Spain and China.

They retrieved the DNA from a thighbone found in a cave in northern Spain.

It is among thousands of fossils from at least 28 individuals to be recovered from a chamber called the ‘‘Pit of the Bones.’’ The remains are typically classified as Homo heidelbergensis (hy-del-ber-GEN’-sis), but not everybody agrees.

The age of the bones has been hard to determine. A rough estimate from analyzing the DNA is around 400,000 years, which supports what Meyer said is the current view of the anthropologists excavating the site. Todd Disotell, an anthropology professor at New York University, said geological techniques suggest the remains are older than 300,000 years but it’s not clear by how much. By comparison, modern humans arose only about 200,000 years ago.

The researchers mapped almost the complete collection of so-called mitochondrial DNA. While the DNA most people know about is found in the nucleus of a cell, mitochondrial DNA lies outside the nucleus. It is passed only from mother to child.

Researchers used the DNA to construct possible evolutionary family trees that include the Spanish individuals and two groups that showed up much later: Neanderthals and an evolutionary cousin of Neanderthals called Denisovans.

They assumed the DNA would show similarities to Neanderthal DNA, since the Spanish fossils have anatomical features reminiscent of Neanderthals.